Fig. 6. Comparison of EGFR and EphA2 interactions with the lipid bilayer.

(A) Snapshots illustrating the predominant conformation of the EGFR-EphA2 heterodimer after molecular dynamics simulations. The intracellular view highlights the EGFR-EphA2 heterodimer’s association with the lipid bilayer, where EGFR is shown in salmon and EphA2 in purple-blue surfaces. Cholesterol, PIP2, and POPS are depicted as cyan, green, and yellow spheres, respectively, while POPC is displayed as gray lines for clarity. The image on the right shows the interaction interface surface of the heterodimer viewed from the intracellular side. (B) Comparison of the number of contacts between EGFR and EphA2 with PIP2 in the membrane over the course of the simulation. The number of contacts is averaged across four replica simulations, considering a 6-Å cutoff distance. KD, kinase domain; SAM, sterile alpha motif.