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. 2024 Dec 4;13:RP96933. doi: 10.7554/eLife.96933

Figure 4. Purification of ScRev7 and ScRev7-C1 proteins.

Figure 4.

(A) SDS–PAGE analysis of protein samples from different stages of ScRev7 purification. Lane 1, standard protein markers; lane 2, uninduced cell lysate (10 μg); lane 3, induced cell lysate (10 μg); lane 4, Ni2+-NTA column eluate (3 μg); lane 5, Superdex S75 column eluate (1 μg); lane 6, eluate from heparin column (0.8 μg). (B) SDS–PAGE analysis of purified truncated ScRev7-C1 variant. Lane 1: standard protein markers. Lanes 2 and 3, purified full-length ScRev7 and ScRev7-C1 variant.

Figure 4—source data 1. Original files for gel images displayed in Figure 4.
Figure 4—source data 2. PDF file containing labelled uncropped gel images displayed in Figure 4.