Figure 8. S. cerevisiae REV7 facilitates chromosomal double-strand break (DSB) repair via non-homologous end-joining (NHEJ) pathway.

(A) A schematic diagram showing the ‘suicide deletion’ cassette at the ADE2 locus of chromosome XV redrawn from Karathanasis and Wilson, 2002. The arrows indicate the locations of PCR primers. Brown and staggered red boxes correspond to direct repeats and I-SceI cleavage sites, respectively. (B) Quantification of NHEJ efficiency in the wild-type YW714 and derivative mutant strains. (C) Representative gel images of PCR-amplified DNA products from Ade2⁺ transformant cells. Data are means ± SEM from three independent experiments. n.s.: not significant, **p < 0.005, ***p < 0.001, ****p < 0.0001 versus control, as assessed by one-way ANOVA Dunnett’s multiple comparison test. The exact p-values are presented in Supplementary file 1d.