The computer-aided design (CAD) of the microfluidic chip with the hydrogel scaffold separating the vascular side (HUVEC) from the channel with the tumor cells (U3013), (a). Magnification of the CAD design to display the details of the hydrogel scaffold and the microfluidic chip components (b). Brightfield, (c), and fluorescent, (d), image of the magenta-labelled U3013 cells (top channel) and green-labelled HUVECs (bottom channel) cultured on the opposite walls of the hydrogel scaffold (Leica SP8, 10× and NA 0,3 objective).