Biocompatibility of 2PP hydrogel for a 2D culture of U3013 cells. Scheme showing the hydrogel disk 3D printing and the U3013 culture protocol (a). The cells were cultured on a hydrogel disk 3D printed inside a microfluidic chip (b), stained for live (c), dead (d), and staining after 24 h of culture (Leica SP8, 10×, NA 0.3 objective) (d). Viability of U3013 cells cultures on the 2PP hydrogel and on the well plate after 24 h of culture (n = 3, error: standard deviation) (e).