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. 2024 Dec 3;8(4):046113. doi: 10.1063/5.0227135

FIG. 6.

FIG. 6.

Tumor invasion comparison of U3013 monoculture and U3013-HUVEC co-culture. Scheme showing the scaffold 3D printing and the cell coculture protocol (a). U3013 cell invasion after 12 h of monoculture (b). U3013 invasion after 12 h of U3013-HUVEC co-culture (Leica SP8, 10× and 0.3 NA objective) (c). The number of cells invading the hydrogel channel after 12 h of cell culture. * corresponds to significant differences between the samples (p < 0.05, n = 18, error: standard deviation) (d). Top (e) and cross section (f); view of GBM cells invading the 40 mW sections in a coculture system after 4 days (Leica SP8, 25× and 0.95NA objective). The arrows indicate GBM filopodia; red: F-actin.