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. Author manuscript; available in PMC: 2024 Dec 4.
Published in final edited form as: Nature. 2023 Jan 18;614(7949):762–766. doi: 10.1038/s41586-022-05626-9

Extended Data Fig. 1 |. Iteratively stimulated T cells populate non-lymphoid tissues.

Extended Data Fig. 1 |

a) CD45.1+ 48° ISTC memory CD8 T cells were transferred to naive mice, followed by three heterologous prime-boost-boost immunizations. Non-lymphoid tissues were analyzed 48 days after the 51° boost. Flow cytometry plots are gated on live CD8a+ lymphocytes that were not stained by intravascular in vivo antibody labeling. b) CD69 expression on endogenous 3° cells (CD45.1-) and 48° memory CD8 T cells (CD45.1+).Plots concatenated from four mice and experiment is representative of three similar experiments with similar results.