Fig. 3.
Intestinal tight junction-related gene expression in largemouth bass fed diets with graded glycyrrhetinic acid (GA) levels for 11 weeks. (A) Zonula occluden-1 (ZO-1), occludin, claudin-1-related mRNA levels expression. (B) The levels of related proteins ZO-1, occludin, and claudin-1. (C) Immunohistochemistry for ZO-1 observed under a fluorescence microscope (scale bar = 100 μm). Bars with different letters indicate a significant difference (P < 0.05).