Figure 2.

Validation of G3PP deletion in both the conditional and tamoxifen (TMX)-inducible G3PP-KO mice. A. Western blot for G3PP expression in whole testis lysates from WT, G3PP^flox/flox, and cG3PP-KO (G3PP^flox/flox-Vasa-Cre) mice. Densitometric analysis for G3PP expression normalized to β-actin is shown in the right panel. B–C. Western blot for G3PP expression in the TMX-inducible G3PP-KO mice. Whole testis protein lysates were prepared from TMX or corn oil (VEH) treated G3PP^flox/flox (Flox-VEH and Flox-TMX) and G3PP^flox/flox-Vasa-CreERT2 (Flox-CreERT2-VEH and Flox-CreERT2-TMX) mice (B); and sperm isolated from the caudal epididymis (C). G3PP expression was normalized to both α-tubulin and β-actin for both testis and sperm Western blots. D. Validation of G3PP deletion in germ cells of cG3PP-KO mice by IHC of testes. G3PP staining was done on testis sections from 9-week-old WT, G3PP^flox/flox control) and KO (G3PP^flox/flox-Vasa-Cre) mice. E. Validation of G3PP deletion in germ cells of icG3PP KO mice by IHC on testis sections. G3PP staining is shown at different levels of magnification. G3PP^flox/flox mice treated with TMX (Flox-TMX) served as the control shown in the upper three panels, and the lower three panels show the KO group (G3PP^flox/flox-Vasa-CreERT2 mice treated with TMX).