Figure 3.

Hypoxia increases basal glycolytic activity

(A) Fluorescence ratio changes of HYlight in primary mouse hepatocytes after 24 h treatment with 1 mM DMOG, 1 h of glucose starvation, and sequential addition of glucose and 2-DG. Data are presented as mean ± SEM. Next, averaged fluorescence ratio upon starvation, and glucose addition. Data are presented as mean ± SEM. Glucose response is expressed as the percentage increase from starvation to glucose addition. Data are presented as mean ± SD. n = 3 with at least 10 cells per replicate.

(B) Fluorescence ratio changes of HYlight in HepG2 cells after 24 h of 1 mM DMOG treatment, 1 h of glucose starvation, and sequential addition of glucose over 35 min. Data shown as mean ± SD. The bar graphs depict the averaged fluorescence ratio upon starvation, and glucose addition. Data are presented as mean ± SEM. Glucose response is expressed as the percentage increase from starvation to glucose addition. Data are presented as mean ± SD. Sample size is n = 5, with at least 50 cells per replicate. ns: Not significant; ∗<0.05; ∗∗<0.005; ∗∗∗<0.0005; ∗∗∗∗<0.00005. Ordinary One-Way ANOVA Šídák's Multiple Comparisons Test.