Figure 4.

HYlight reacts with fructose 1,6-bisphosphate (FBP) and dihydroxyacetone phosphate (DHAP)

(A) HYlight was tested toward several metabolites from different metabolic pathways.

(B) Comparison of the ratio change in HYlight upon different metabolites at physiological concentrations. ns: Not significant; ∗<0.05; ∗∗<0.005; ∗∗∗<0.0005; ∗∗∗∗<0.00005. Ordinary One-Way ANOVA Šídák's Multiple Comparisons Test. Data are presented as mean ± SD. n = 3. GTP: guanosine-5′-triphosphate (1 mM); GDP: guanosine diphosphate (1 mM); glucose 1-phosphate (1 mM); 6-phosphogluconate (0.5 mM); α-ketoglutarate (0.79 mM); succinate (6.8 μm); succinyl-CoA (6.8 μM); pyruvate (16 μM); oxaloacetate (2 μM); malate (1.39 mM); fumarate (0.38 mM); citrate (32 μM); acetyl-CoA (28 μM); xilose (5 mM), ribose (5 mM); maltose (5 mM); manose (5 mM); lactose (5 mM); glucose (5 mM); fructose (5 mM); ATP (4.67 mM); NADPH (65 μM); NADH (75 μM); NAD+ (0.5 mM); 3PG: 3-phosphoglycerate (0.37 mM); 2,3-BPG: 2,3-bisphosphoglycerate (0.23 mM); G3P: glyceraldehyde 3-phosphate (0.14 mM); DHAP (1.63 mM); FBP (1.52 mM); phosphoenolpyruvate (16 μM); G6P: glucose 6-phosphate (0.67 mM); F6P: fructose 6-phosphate (96 μM); SEC: size exclusion chromatography buffer: 50 mM Tris pH 7.5; 125 mM NaCl. Sample size is n = 3. See also Figure S3 for additional details. Figure 4A can also be accessed through BioRender: BioRender.com/b12m469.