Table 2.
Example of Jess protocol
| Value | |
|---|---|
| Separation Matrix | |
| Well Row | L1 |
| Load Time (sec) | 200.0 |
| Stacking Matrix | |
| Well Row | M1 |
| Load Time (sec) | 12.0 |
| Sample | |
| Well Row | A1 |
| Load Time (sec) | 6.0 |
| Separation Time (min) | 30.0 |
| Separation Voltage (volts) | 375 |
| Standards Exposure (sec) | 4.0 |
| EE Immobilization Time (sec) | 200.0 |
| Protein Normalization (PN) Time (min) | 25.0 |
| Well Row | B1 |
| Antibody Diluent Time (min) | 5.0 |
| Well Row | C1 |
| Primary Antibody Time (min) | 30.0 |
| Well Row | D1 |
| Secondary Antibody Time (min) | 30.0 |
| Well Row | E1 |
| Detection | |
| Well Row | F1 |
| Detection Profile (Chemi) | HDR |
| Detection Profile (NIR) | None |
| Detection Profile (IR) | None |
| Ladder Channel | CHEMI |