Table 3.

Jess machine run step-by-step overview

Step	Description
1	Separation and stacking matrixes are pre-aspirated in a capillary
2	Proteins in a capillary are separated according to their MW
3	Proteins are immobilized by UV light to a capillary wall
4	Clearing of the matrix from the capillary step
5	Initiation of immunoprobing process: incubation with primary antibody, secondary HRP conjugate and chemiluminescent substrate
6	The emitted chemiluminescent light is recorded by CCD camera and then quantified