Table 3.
A comparison of the present study with earlier research regarding the different methods employed to extract and purify C-PC from various cyanobacteria.
| Strain | Extraction | Purification method | References | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Method | C-PC concentration (mg mL−1) | C-PC purity ratio | Yield (mg C-PC g DCW−1) | Method | C-PC concentration (mg mL−1) | C-PC purity ratio | Yield (mg C-PC g DCW−1) | ||
| Desertifilum tharense UAM-C/S02 | repeated FT | 0.822 | 1.40 | 86.46 | Two-step ASP+ Dialysis | 0.576 | 3.83 | ND | Hernández Martínez et al. (2023) |
| Synechocystis sp. | BB | ND | 1.15 | 182.4 | AEC | ND | 4.84 | 16.9 | Avci and Haznedaroglu (2022) |
| Phormidium sp. | FT | ND | 0.55 | 48.1 | ASP | ND | 1.75 | 33.0 | Avci and Haznedaroglu (2022) |
| Euryhalinema sp. | Ultrasonication + FT | ND | 0.76 | 15.02 | ASP + Sephadex-G25 + DEAE-Sephadex | ND | 4.1 | ND | Khazi et al. (2021) |
| Leptolyngbya sp. | MP + three cycles of FT | 0.038 | 0.36 | ND | Three-stage ASP + AEC | 0.092 | 3.5 | ND | Prabha et al. (2023) |
| Synechocystis sp. PCC 6803 | High pressure homogenisation | ND | 1.25 | 75.3 | Two-step ASP + heat-treatment | ND | 2.9 | ND | Puzorjov et al. (2022) |
| Spirulina sp. | The enzyme treatment | 0.081 | 0.93 | ND | ASP + Dialysis + DEAE column chromatography | 0.545 | 5.02 | ND | Mahendran et al. (2022) |
| Arthrospira platensis | [EMIM][EtSO4] ionic liquid + sonication | ND | 0.54 | 76.6 | dialysis + precipitation | ND | 3.5 | 48.9 | Sánchez-Laso et al. (2023) |
| Spirulina | 3 cycles of FT | ND | 0.77 | 217.18 | - | - | - | - | Chentir et al. (2018) |
| Spirulina | 4 cycles of FT | ND | 0.66 | 73.73 | - | - | - | - | Tavanandi et al. (2018) |
| Spirulina | Soaked in potassium phosphate buffer | ND | 0.93 | 17.2 | chitosan + activated charcoal + chromatography on DEAE Sephadex A-25 | ND | 4.30 | ND | Liao et al. (2011) |
| Cyanobium sp. MMK01 | 1 cycles of FT | 6.5 | 1.86 | 225 | Two-step ASP | 3.85 | 4.04 | 120 | This study |
| Cyanobium sp. MMK01 | 1 cycles of FT | 6.5 | 1.86 | 225 | Two-step ASP +IEC | 0.34 | 5.82 | 6.8 | This study |
FT, Freeze/thaw Cycles; BB, Bead-beating; MP, mortar and pestle; ASP, ammonium sulfate precipitation; AEC, Anion-exchange chromatography; IEC, ion exchange chromatography; DEAE, diethyl aminoethyl; HPLC, high-performance liquid chromatography. ND: Not determined. *Phycocyanin purity index: A615-620/A280 > 0.7—food grade; >1.5—cosmetic grade, >3.9—reagent grade, >4.0—analytical grade.