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. 2024 Dec 5;28:0115. doi: 10.34133/bmr.0115

Fig. 5.

Fig. 5.

Multicellular self-assembly of 3D bioprinted CBV constructs. (A and B) Postculture 3D reconstructed confocal microscopy images showing multicellular self-assembly of the printed CBV constructs: HBMECs (VE-cadherin, green) and HBVPs (platelet-derived growth factor receptor beta [PDGFR-β], red); scale bars: 200 μm. (C) Schematic representation of HBMEC and HBVP compartmentalization. (D) Fluorescence intensity plot of the cells in the white box in (B), indicating the compartmentalization of HBVPs (red) and HBMECs (green). (E) Filamentous actin (F-actin) and platelet endothelial cell-adhesion molecule-1 (PECAM-1) expression in mature CBV constructs (scale bars: 200 μm). (F) Morphology of junctional proteins (scale bar: 40 μm) in mature CBV constructs. (G) Permeability assay of the CBV constructs cast in PEVA chambers using fluorescein isothiocyanate (FITC)-labeled dextran. (H) Permeability differences between the bare, HBMEC-only, and HBMEC + HBVP self-assembled constructs (green: 40-kDa FITC–dextran; scale bars: 200 μm). (I) Calculated diffusional permeability values for the constructs shown in (H). Data represent mean ± SEM (n = 2, 3, and 4 respectively; *P < 0.05; unpaired Student t test).