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. 2024 Dec 5;28:0115. doi: 10.34133/bmr.0115

Fig. 6.

Fig. 6.

Neuroinflammatory responses observed with the cerebral blood vessel model. (A) Immunofluorescence staining showing junctional down-regulation during inflammation (scale bars: 20 μm). (B) Quantification of the total area of neuroinflammatory cytokine (RANTES) expression and adhesion molecule (intercellular adhesion molecule 1 [ICAM-1]) expression for the samples presented in (A). Data represent mean ± SEM (n = 3 per group; *P < 0.05; one-way ANOVA). (C and D) Up-regulation of RANTES (C) and ICAM-1 (D) after inducing inflammation in cerebral blood vessel constructs. Data represent mean ± SD (*P < 0.05; **P < 0.01; ***P < 0.001; one-way ANOVA). (E) Permeability assay: increased abluminal diffusion of FITC-labeled dextran during neuroinflammation of cerebral blood vessel constructs (green: 40-kDa FITC–dextran; scale bars: 200 μm). (F) Calculated diffusional permeability of the samples shown in (E), indicating that neuroinflammation increased permeability (10-kDa FITC-labeled dextran). Data represent mean ± SD (n = 3 per group; *P < 0.05; unpaired Student t test).