Figure 9.

Free-energy along the MFEPs for the HD zipping shown in Fig. 8. Free energies are shown (A) as a function of, α, the order parameter derived from the string method and (B) ${\theta }_{CH}$, the angle through which the HD has “zipped.” ${\theta }_{CH}$ is only calculated while the HD is zipping, i.e., the data used in (B) is cut off at the more complicated final rupture of the outer membrane. Data are shown for (dashed) $\sigma =0.129\kappa /d^2$ and (solid) $\sigma =0.387\kappa /d^2$, free energies are given relative to that of a dNT, $F_{\text{dt}}$ and key steps are illustrated for $\sigma =0.129\kappa /d^2$.