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(A) Microscope was utilized to observe the BMSCS characteristics. (B) Flow cytometric sorting used CD45(−), CD90(+) and CD29(+) as sorting criteria. (C) Nano-flow results to present the extracted precipitate characteristics. (D) Electron microscopy of the precipitate showed a teacup mouth shape morphology. (E) WB results show that the precipitate expresses TSG101 and CD63, but not Calnexin. (F) Cytosolic precipitate attached to the wall of the centrifuge tube.
