Fig. 4.

(A) Alizarin Red S staining and ALP staining results. (B) Western blot analysis detected the expression levels of osteogenesis-related proteins. (C) Analysis of grayscale values of bands corresponding to osteogenesis-related proteins. (D) qRT-PCR detected the mRNA expression levels of osteogenic-related genes. (E) Establishment of a rat cranial defect model and analysis of experimental results. (F) Histological results of HE staining. ∗, p < 0.05; ∗∗, p < 0.01; ∗∗∗, p < 0.001; Mean ± SEM.