Fig. 4.

Hippo/YAP signaling pathway regulated the expression of ACSL4 and ferroptosis in pericytes. (A) KEGG pathway (downregulated) of differentially expressed genes between control groups and LPS treated groups. (B) Immunofluorescence analysis of the nuclear translocation of YAP1. (C) Western blotting analysis of YAP1 of pericytes after LPS treatment. (D) The relative expression level of YAP1 after treatment in Si-NC and Si-YAP1 of pericytes. (E) The relative expression level of ACSL4 after treatment in Si-YAP1 of pericytes. (F) Cell viability of LPS-stimulated pericytes pre-treated with Si-YAP1. (G) The levels of lipid peroxidation, GSH/GSSG ratio, GSH and ferrous iron of LPS-stimulated pericytes pretreated with Si-YAP1.

For in vitro experiments or cell samples, n = 3 for each group. Data were shown as mean ± SD. ∗p < 0.05, ∗p < 0.01, ∗∗∗p < 0.001. Statistical significance was determined by one-way ANOVA or two-sided Student's-test as appropriate.