Fig. 5.

Pericytes-specific ACSL4 knockout improved pulmonary vascular barrier function in sepsis. (A) Representative images of immunofluorescence co-localization of ACSL4 and NG2 in the mouse lung tissues. (n = 8/group) (B) Representative images of Evens-Blue leakage in the lung tissues. (n = 6/group) (C) Flow cytometry analyses of ferroptosis of CD31⁻CD140b + cells (pericytes) in the lung of mice. (n = 8/group) (D) Laser confocal microscopy analysis of the pulmonary networks. (NG2, green, pericyte marker; CD31, red, endothelial cell marker. Bar = 50 μm). (n = 8/group) (E) Western blotting analysis of pericyte marker (PDGFR-β and NG2) in the lung tissues. (n = 3/group) (G) Survival curves of rats undergoing sham and CLP surgery. (n = 16/group)

Data were shown as mean ± SD. ∗∗∗p < 0.001. Statistical significance was determined by one-way ANOVA as appropriate. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)