Fig. 6.

Pericytes targeting liposomes encapsulating ACSL4 shRNA adenovirus inhibited pericytes ferroptosis and improved pulmonary vascular barrier function. (A) Flow cytometry analyses of ferroptosis of CD31⁻CD140b + cells (pericytes) in the lung of mice. (n = 8/group) (B) Laser confocal microscopy analysis of the pulmonary networks. (NG2, green, pericyte marker; CD31, red, endothelial cell marker. Bar = 50 μm). (n = 8/group) (C) Representative images of Evens-Blue leakage in the lung tissues. (n = 6/group) (D) Western blotting analysis of pericyte marker (PDGFR-β and NG2) in the lung tissues. (n = 3/group) (E) Representative images of FITC-BSA leakage in the lung tissues. (Blue, DAPI; green, FITC-BSA. Bar = 50 μm) (F) Inflammatory factors levels (IL-6 and TNF-α) in serum. (n = 8/group) (G) Survival curves of rats undergoing sham surgery, CLP and Fer-1 liposomes pretreatment. (n = 16/group)

Data were shown as mean ± SD. ∗p < 0.05, ∗∗∗p < 0.001. Statistical significance was determined by one-way ANOVA as appropriate. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)