Table 1.

PCR Reaction & Cycling
Acsl4-Flox
Primer	Sequence (5’→3′)			Primer Type
P1	AGTTAGCAGAGGGAGGCTGA			Forward
P2	GGCTTGCTTGTGGCACATTA			Reverse
PCR Reaction System	Reaction Component			Volume (μl)
	ddH2O			7.0
	2 × transTaq-T PCR SuperMix			10.0
	P1 (10 pmol/μl)			0.5
	P2 (10 pmol/μl)			0.5
	Genomic DNA（50–100 ng/μl）			2
	Total			20
	2 × transTaq-T PCR SuperMix from TransGen Biotech (Code number:AS122)
Cycling Reaction	Step	Temp	Time	Note
	1	94 °C	3 min
	2	94 °C	30 s
	3	60 °C	30 s
	4	72 °C	30 s	34 repeats to 2
	5	72 °C	5 min
	6	12 °C	Hold
Genotype	Wild type：one band with 318 bp
	Heterozygote：two bands with 318 and 372 bp
	Homozygote：one band with 372 bp

Pdgftb-creERT2
Primer	Sequence (5’→3′)			Primer Type
P3	GAACTGTCACCGGGAGGA			Transgene Forward
P4	AGGCAAATTTTGGTGTACGG			Transgene Reverse
P5	CAAATGTTGCTTGTCTGGTG			Internal Positive Control Forward
P6	GTCAGTCGAGTGCACAGTTT			Internal Positive Control Reverse
PCR Reaction System	Reaction Component			Volume (μl)
	ddH2O			6.0
	2 × transTaq-T PCR SuperMix			10.0
	P3 (10 pmol/μl)			0.5
	P4 (10 pmol/μl)			0.5
	P5 (10 pmol/μl)			0.5
	P6 (10 pmol/μl)			0.5
	Genomic DNA（50–100 ng/μl）			2
	Total			20
	2 × transTaq-T PCR SuperMix from TransGen Biotech (Code number:AS122)
Cycling Reaction	Step	Temp	Time	Note
	1	94 °C	3 min
	2	94 °C	30 s
	3	60 °C	30 s
	4	72 °C	30 s	34 repeats to 2
	5	72 °C	5 min
	6	12 °C	Hold
Genotype	Tg：∼400 bp
	Control：200 bp