Fig. 1.

Tumor cell cytokines induce monocyte migration in Ccr2-and Ccr1-dependent manner. A, Bio-Plex analysis of chemokines in conditioned medium of LLC1.1 (left) and MC38GFP (right) -wt and -Ccl2KD cells. Data is shown as pg of chemokine per ml of medium. n = 6. B-D, Migration of BM-derived CD115⁺ monocytes from BL6 (grey), Ccr1^-/- (orange) or Ccr2^-/- (blue) mice towards Ccl2, Ccl5 or both chemokines (B); CM of LLC1.1 and MC38-GFP wt cells (C) and CM of LLC1.1 wt and Ccl2KD cells and CM of MC38-GFP wt and Ccl2KD cells, respectively (D). Data was normalized to the number of migrated monocytes in either DMEM/2 % h.i. FBS (B-C) or in the wt CM (D). Two independent experiments, n = 3-4.