Fig. 4.

Intravenous injection of Ccr2-deficient but not Ccr1-deficient monocytic cells restores lung metastasis. A, Quantification of metastatic foci in an experimental metastasis model after the injection of LLC1.1-wt tumor cells into BL6 or Ccr1^-/- mice followed by an adoptive transfer (AT) of BM-derived CD115⁺ monocytic cells isolated from BL6, Ccr1^-/-, or Ccr2^-/- mice and terminated on day 15. n=5. B, Spontaneous lung metastasis of LLC1.1-wt cells. The subcutaneous primary tumor was removed on day 14 and AT of CD115⁺ cells was i.v. injected at 1, 3 and 5 days after tumor removal. Mice were terminated 21 days post-tumor removal. Quantification of metastatic foci (right panel) with representative pictures of metastatic lungs (left panel). Scale bar, 5 mm; n = 4–5. C, Quantification of metastatic foci in an experimental metastasis model of LLC1.1-wt tumor cells injected in chimeric Ccr2^-/-→BL6 mice, followed by an AT with CD115⁺ cells isolated from BL6, Ccr1^-/- or Ccr2^-/- mice was performed. BL6→BL6 mice were used as a control. n = 5–12. D, Quantification of metastatic foci in an experimental lung metastasis model of LLC1.1 wt cells injected in chimeric mice (Ccr1^-/-→BL6) followed by AT of CD115⁺ cells isolated from BL6 or Ccr2^-/- mice (left panel) with representative pictures (right panel). n=5-6.