Fig. 5.
The effect of LHNTMZ, LHNrutin and LHNTMZ+rutin on GB tumor growth. (A) Generation of an allograft GB model and insertion of LHN webs. (a) A midline skin incision exposed the right frontal intracranial surfaces. (b) The dura was opened following a 5 mm x 5 mm right frontal craniectomy. (c) Injected 2.5 × 106/10 µl of C6 cell into 2 mm lateral and anterior to bregma. (d, e) LHN webs were inserted into the resected area. (f) A bone wax was used to cover the resection area. (B) Coronal T2 weighted and axial T2 weighted MR scans on Day 11. UNT GB rats: Tumor lesion causing infiltrative edema in the right frontal deep white matter (yellow arrow) and hydrocephalus secondary to compression (blue arrow). After LHNTMZ: a slightly hyperintense infiltrative tumoral lesion (yellow arrow) in the right frontal region extends transcallosally from the anterior part of the corpus callosum, with the LHN network observed at its center (red arrow). After LHNrutin: In the deep white matter of the right frontal lobe, tumor lesion (yellow arrow) and a prominent hypointense large-sized nanofiber (red arrow) are observed. The lesion extends transcallosally to the opposite hemisphere, exerting pressure on the LHN network. After LHNTMZ+rutin: Hypointense LHN (red arrow) in the right frontal area and a small tumoral lesion of a millimetric thickness (yellow arrow) surrounding it. (C) The appearance of the resected brain and the tumor at the end of the experiment. (D) H&E staining of brain sections on Day 11. (E) The volume of the GB tumors. *P < 0.05.