Extended Data Fig. 3. Additional examples of trajectories relating to the experiment described in Fig. 2 of the main text, and exchange of a heterotypic nucleosome.

a, & b, Single exchange events, where the exchange event is preceded by SWR1 binding. c, & d, Processive double exchange events. A single SWR1 binding event is followed by two consecutive exchange events. (Data in (c) is from Fig. 2d of the main text, replotted here to additionally show the donor and acceptor trajectories.) e, Distributive double exchange event. Following the first exchange SWR1 dissociates. The second exchange is preceded by a SWR1 binding event. f, Ambiguous double exchange example. SWR1 either dissociates or photobleaches between the first and second exchange events. g, Schematic of three-color smFRET assay using a heterotypic nucleosome as the substrate. Schematic is colored similarly to Fig. 2a of the main text. h, & i, Example trajectories showing SWR1 binding and histone exchange of a heterotypic nucleosome substrate. j, Histogram showing the FRET before (white bars) and after (grey bars) exchange, for a heterotypic nucleosome. k, Distribution of the time between SWR1 binding and histone exchange yields an exchange time of 106 ± 5 s for a heterotypic nucleosome. Reported errors are the error of the fit.