Extended Data Fig. 10. Long-term reconstitution potential of skull HSCs.
a. Diagram showing experimental scheme for serial hematopoietic reconstitution following serial partial irradiation with shielding. b. Monthly FACS analysis of T, B, and myeloid cells in peripheral blood isolated from animals with head versus leg shielding during primary and secondary hematopoietic reconstitution (n = 5 (Head shield), n = 4 (Leg shield) mice/group from two independent experiments). Vertical bars indicate mean ± SD. c-e. Fluorescence imaging (c) and quantification (d, e) of skull or femoral BM blood vessels in Cdh5-mTnG (mTomato, red) reporter mice at 7 days after lethal irradiation with (+) or without (-) bone marrow transplantation (BMT), as indicated. Note minimal vascular alterations in the skull BM compared to substantial vascular changes in femoral BM (n = 6 mice/group for vascular area/density, n = 10 randomly selected vessels from all samples/group for vessel diameter from three independent experiments). Vertical bars indicate mean ± SD. P values were calculated using Tukey multiple comparison test (one-way ANOVA).