Extended Data Fig. 3. Regional differences and expansion of skull BM.
a, c. In vivo IF staining (a) and quantification of BM vessels (c) in Flk1-GFP reporter mice showing distinct blood vessel architecture in frontal (F), parietal (P) and interparietal (i-P) skull. Arrowheads indicate Flk1low/− CD31+ arterioles. n = 6 mice/group for vascular area, n = 20 randomly selected vessels from all samples/group for vessel diameter from two independent experiments. Boxed areas in overview image (left) indicate location of magnified images. Scale bar, 1 mm. b, d. Imaging (b) and quantification (d) of Evans Blue vascular leakage in different skull bone parts (n = 4 mice/group from three independent experiments). Boxed areas in overview image (left) indicate location of magnified images. Scale bar, 1 mm. e. IF staining of skull BM blood vessels showing increase in vascular network complexity with aging. Dotted lines demarcate dorsal and ventral skull boundaries. Representative images from four independent experiments. Scale bars, 100 μm. f, g. IF staining and quantification of caveolin-1-positive (Cav1+) blood vessels showing an increase with aging (n = 8 mice/group from two independent experiments). Dotted lines demarcate dorsal and ventral boundaries of the skull. Scale bars, 100 μm. Vertical bars indicate mean ± SD. P values were calculated using Tukey multiple comparison test (one-way ANOVA) and two-tailed unpaired Student’s t-test.