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. 2024 Dec 4;15:10566. doi: 10.1038/s41467-024-54780-3

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a–d Flow cytometric plots and quantification of B1 cells in (a), the peritoneal lavage (Il33^+/+ n = 7, Il33^−/− n = 7), b the thoracal lavage (Il33^+/+ n = 6, Il33^-/- n = 4), c omentum (Il33^+/+ n = 6, Il33^−/− n = 3) and (d) lung (Il33^+/+ n = 5, Il33^-/- n = 3) of Il33^-/- and control mice. e, f Flow-cytometric analysis of Ki-67 of B1 cells (Pre-gated on Live CD45⁺ CD19⁺ CD23⁻) in Il33^−/− versus control mice in the (e), peritoneal lavage (Il33^+/+ n = 5, Il33^-/- n = 4) and the (f) thoracal lavage (Il33^+/+ n = 4, Il33^−/− n = 3). g–j Flow-cytometric plots and quantification of B1 cells of Nmur1^iCre-eGFP Il1r1^fl/fl (ST2) mice and littermate controls in (g), the peritoneal lavage (Il1rl1^fl/fl n = 4, Nmur1^iCre-eGFP Il1rl1^fl/fl n = 5) h the thoracal lavage (Il1rl1^fl/fl n = 4, Nmur1^iCre-eGFP Il1rl1^fl/fl n = 4), i omentum (Il1rl1^fl/fl n = 4, Nmur1^iCre-eGFP Il1rl1^fl/fl n = 4), and (j) lung (Il1rl1^fl/fl n = 4, Nmur1^iCre-eGFP Il1rl1^fl/fl n = 4). k Sort-purified small intestinal ILC2s gated as Live CD45⁺ Lin⁻ (CD3, CD5, CD19, Fcεrl, Ly6G) CD127⁺ KLRG1⁺ of Tslpr^−/− (Tslpr^+/+ n = 5, Tslpr^−/− n = 5), Il17rb^−/− (Il17rb^+/+ n = 5, Il17rb^−/− n = 4)⁻, Il33^−/− (Il33^+/+ n = 5, Il33^−/− n = 5) and Nmur1^iCre-eGFP Il1rl1^fl/fl (Il1rl1^fl/fl n = 6, Nmur1^iCre-eGFP Il1rl1^fl/fl n = 7) and qPCR for Il5. Each symbol represents data from one mouse, mean +/− SD, data are representative of at least two independent experiments. Statistical significance was determined by two-tailed unpaired Student’s t-test (a–j) or Mann Whitney test (k), n.s. non-significant, *p < 0.05, **p < 0.01, ***p < 0.001. Source data, including exact p-values, are provided as a Source data file Fig. 6.