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. 2024 Sep 17;31(12):1717–1729. doi: 10.1038/s41418-024-01379-2

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Representative flow cytometry plots showing identification of TIM4− and TIM4+ macrophages (F4/80+ CD11b+) in C57B6/J and Nlrp3^−/− (Nlrp3^tm1Flv) mouse peritoneum. Cells were pre-gated on single, live and CD45+ populations. b Bar plot of the total number of macrophages (CD11b+ F4/80+) in each peritoneum of each mouse (Nlrp3^−/−, n = 4; C57B6/J, n = 4). P value was calculated using an unpaired t-test. Error bars represent standard deviation. c Representative histogram of distribution of TIM4− and TIM4+ macrophages (F4/80+ CD11b+) in C57B6/J and Nlrp3⁻^/⁻ (Nlrp3^tm1Flv) mouse peritoneum. d Bar plot of TIM4− and TIM4+ macrophages (F4/80+ CD11b+) in C57B6/J, Nlrp3^−/− (Nlrp3^tm1Flv) and Casp1/11^−/− mouse peritoneum. Percentages above bars show the mean value for each group. (Nlrp3⁻^/−, n = 7; C57B6/J, n = 7; Casp1/11^−/−, n = 8). Adj. P values were calculated using a two-way ANOVA with Turkey’s multiple comparison testing. *adj. P < 0.05, **adj. P < 0.01, ***adj. P < 0.001, ****adj. P < 0.0001, ns not significant (adj. P > 0.05). Error bars represent standard deviation. e Bar plot of TIM4− and TIM4+ macrophages (F4/80+ CD11b+) in C57B6/J and 129S2 mouse peritoneum. Percentages above bars show the mean value for each group (C57B6/J, n = 4; 129S2, n = 4). P values were calculated using multiple unpaired parametric t-tests. FDR (q) was calculated using Benjamini–Hochberg correction. *q < 0.05, **q < 0.01, ***q < 0.001, ****q < 0.0001, ns not significant (q > 0.05). Error bars represent standard deviation. f log₂ normalised counts of Timd4 from RNAseq of Nlrp3⁻^/− and Nlrp3^+/+ (Nlrp3^tm1Flv) BMDMs at baseline and after 3 and 24 h of LPS (10 ng/ml) stimulation (Nlrp3⁻^/−, n = 4; Nlrp3^+/+, n = 4). *adj. P < 0.05, **adj. P < 0.01, ***adj. P < 0.001, ****adj. P < 0.0001, ns not significant. Wald Test, Benjamini–Hochberg corrected.