Skip to main content
Biochemical Journal logoLink to Biochemical Journal
. 1980 May 15;188(2):475–479. doi: 10.1042/bj1880475

A comparison of erythrocyte glutathione S-transferase activity from human foetuses and adults.

R C Strange, J D Johnston, D R Coghill, R Hume
PMCID: PMC1161891  PMID: 7396875

Abstract

Glutathione S-transferase activity was measured in partially purified haemolysates of erythrocytes from human foetuses and adults. Enzyme activity was present in erythrocytes obtained between 12 and 40 weeks of gestation. The catalytic properties of the enzyme from foetal cells were similar to those of the enzyme from adult erythrocytes, indicating that probably only one form of the erythrocytes enzyme exists throughout foetal and adult life.

Full text

PDF
475

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bass N. M., Kirsch R. E., Tuff S. A., Marks I., Saunders S. J. Ligandin heterogeneity : evidence that the two non-identical subunits are the monomers of two distinct proteins. Biochim Biophys Acta. 1977 May 27;492(1):163–175. doi: 10.1016/0005-2795(77)90223-9. [DOI] [PubMed] [Google Scholar]
  2. Bernard B., Preston B., Beaupre P., Elliott K., Sadava D. Multiple forms of thymidine kinase during human development. Biol Neonate. 1977;31(3-4):225–228. doi: 10.1159/000240964. [DOI] [PubMed] [Google Scholar]
  3. Chasseaud L. F. Distribution of enzymes that catalyse reactions of glutathione with alpha beta-unsaturated compounds. Biochem J. 1973 Apr;131(4):765–769. doi: 10.1042/bj1310765. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chasseaud L. F. The role of glutathione and glutathione S-transferases in the metabolism of chemical carcinogens and other electrophilic agents. Adv Cancer Res. 1979;29:175–274. doi: 10.1016/s0065-230x(08)60848-9. [DOI] [PubMed] [Google Scholar]
  5. Dutton G. J. Developmental aspects of drug conjugation, with special reference to glucuronidation. Annu Rev Pharmacol Toxicol. 1978;18:17–35. doi: 10.1146/annurev.pa.18.040178.000313. [DOI] [PubMed] [Google Scholar]
  6. Habig W. H., Pabst M. J., Jakoby W. B. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem. 1974 Nov 25;249(22):7130–7139. [PubMed] [Google Scholar]
  7. Hayes J. D., Strange R. C., Percy-Robb I. W. Identification of two lithocholic acid-binding proteins. Separation of ligandin from glutathione S-transferase B. Biochem J. 1979 Sep 1;181(3):699–708. doi: 10.1042/bj1810699. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jakoby W. B. The glutathione S-transferases: a group of multifunctional detoxification proteins. Adv Enzymol Relat Areas Mol Biol. 1978;46:383–414. doi: 10.1002/9780470122914.ch6. [DOI] [PubMed] [Google Scholar]
  9. Juchau M. R., Namkung M. J. Studies on the biotransformation of naphthalene-1,2-oxide in fetal and placental tissues of humans and monkeys. Drug Metab Dispos. 1974 Jul-Aug;2(4):380–385. [PubMed] [Google Scholar]
  10. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  11. Marcus C. J., Habig W. H., Jakoby W. B. Glutathione transferase from human erythrocytes. Nonidentity with the enzymes from liver. Arch Biochem Biophys. 1978 Jun;188(2):287–293. doi: 10.1016/s0003-9861(78)80011-3. [DOI] [PubMed] [Google Scholar]
  12. Morgenstern R., DePierre J. W., Ernster L. Activation of microsomal glutathione S-transferase activity by sulfhydryl reagents. Biochem Biophys Res Commun. 1979 Apr 13;87(3):657–663. doi: 10.1016/0006-291x(79)92009-6. [DOI] [PubMed] [Google Scholar]
  13. Mulivor R. A., Hannig V. L., Harris H. Developmental change in human intestinal alkaline phosphatase. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3909–3912. doi: 10.1073/pnas.75.8.3909. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Strange R. C., Cramb R., Hayes J. D., Percy-Robb I. W. Partial purification of two lithocholic acid-binding proteins from rat liver 100 000g supernatants. Biochem J. 1977 Sep 1;165(3):425–429. doi: 10.1042/bj1650425. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Taylor A. T., Stafford M. A., Jones O. W. Properties of thymidine kinase partially purified from human fetal and adult tissue. J Biol Chem. 1972 Mar 25;247(6):1930–1935. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES