Fig. 3. PEGylation of QDs markedly reduces QD-mediated increases in platelet activation. Washed platelets at 1 × 10⁷ mL⁻¹ were incubated with monoclonal antibodies against P-selectin, CD42b, and PAC1 for 5 min before the addition of either QD-LA, QD-LA-PEG, QD-Pen, or QD-Pen-PEG at either 30 nM or 100 nM for 20 min before fixation with PFA and analysis by flow cytometry. Number of platelets positive for (A) and (D) PAC1, (B) and (E) P-selectin, and (C) and (F) QDs are shown (n = 7). Data is shown as mean ± SD. Statistical significance was calculated using a two-way ANOVA followed by a Sidak's multiple comparison post hoc test. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.