Fig. 5. Platelets incubated with QDs are still able to express activation markers in response to thrombin. Washed platelets at 1 × 10⁷ mL⁻¹ were incubated with monoclonal antibodies against P-selectin, CD42b, and PAC1 for 5 min before the addition of either QD-LA, QD-LA-PEG, QD-Pen, or QD-Pen-PEG (at either 30 nM or 100 nM) for 20 min. 0.1 U mL⁻¹ thrombin (Thr) was used to stimulate platelets. Platelets were fixed after 10 min with 0.45% PFA before being analysed by flow cytometry. The number of platelets positive for (A) and (D) PAC1, (B) and (E) P-selectin and (C) and (F) QDs are shown. Data is shown as mean ± SD. n = 7. Statistical significance was calculated using a two-way ANOVA followed by a Sidak's multiple comparison post hoc test. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.