A) Schematic of the Pavlovian conditioned alcohol seeking task, where a CS+ cue predicts alcohol delivery into a reward port, and a CS− cue has no associated outcomes. B, D, F, H) Across training acquisition, rats in the ArchT and YFP-only viral groups learned to discriminate the alcohol predictive CS+ from the CS−. C) Port entry probability, E) number of port entries, F) port entry latency (s), H) and port time (s), differed by cue type in each viral group by the end of training. Data presented as mean +/− SEM. Dots represent individual animals.