Fig 3.
Expression of PilAPA14 restores phage-susceptibility of the PA14ΔpilA mutant. (a) ZC01 and ZC03 phage lysates at the indicated titers (108–105 PFU/mL) were spotted on PA14 and on PA14ΔpilA expressing pilAPA14 or pilAPAO1 upon induction with arabinose. Photographs were taken after overnight incubation at 37°C. (b) Twitching motility assays of wild-type and complemented strains induced or not with arabinose were analyzed by staining the plates with 0.1% crystal violet after 48 h. Images show a representative experiment of at least three independent biological replicates.