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. 2024 Oct 29;12(12):e01527-24. doi: 10.1128/spectrum.01527-24

Fig 4.

Bacterial lysis assays with phages applied at various concentrations. Different bacterial strains and conditions, with or without arabinose, exhibit varying lysis patterns. Bacterial growth zones below indicate impact on phage sensitivity.

Infection of P. aeruginosa PAO1 and PAO1 ΔpilA expressing PilAPA14. (a) ZC01 and ZC03 phage lysates at the indicated titers (109–105 PFU/mL) were spotted on PAO1 or PAO1ΔpilA expressing PilAPA14 upon induction using arabinose. Photographs were taken after overnight incubation at 37°C. (b) Twitching motility assays of wild-type and complemented strains induced or not with arabinose were analyzed by staining the plates with 0.1% crystal violet after 48 h. Images show a representative experiment of at least three independent biological replicates.