Fig. 4.

Expression analysis of BmPOUM2 and WCP genes in the WT and Bmlark^−/− mutant embryos. A Expression profiles of BmPOUM2 and WCP genes in WT embryos at 144, 156, 168, and 180 h post oviposition. Hours post oviposition are indicated in the figure. B RT-qPCR analysis of BmPOUM2 expression in the Bmlark^−/− mutant and WT embryos. C RT-qPCR analysis of the expression of WCP genes in the Bmlark^−/− mutant and WT embryos. Student’s t-test was used to evaluate statistical significance. Results are presented as the mean ± SE of three biological replicates. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001