Table 3.
Average quality (measured by the A260/280ratio; in NanoDrop™), concentration ([DNA]; measured in Qubit Fluorometer), and amount of DNA extracted from Cerastoderma edule gut mass, are presented along with the volume of elution buffer used per extraction kit. Values are presented as mean ± standard deviation. Different letters (in superscript) highlight kits showing significantly different performance in terms of DNA concentration and recovery from gut samples, as per the one-way ANOVA outcome (P < 0.05; cf. Table S2).
| DNA extraction kits | Sample mass used (mg) | Elution buffer (μL) | A260/280 ratio | [DNA] (ng μL−1) | Amount DNA/mg gut (ng mg −1) |
|---|---|---|---|---|---|
| E.Z.N.A. | 200.0 ± 81.65 | 50 | 1.91 ± 0.02 | 62.1 ± 22.03a | 19.2 ± 14.72a |
| FastDNA | 200.0 ± 00.00 | 100 | 1.70 ± 0.12 | 120.3 ± 37.04b | 60.2 ± 18.52b |
| DNeasy | 228.3 ± 30.08 | 50 | 1.90 ± 0.02 | 66.9 ± 6.86a | 15.2 ± 2.62a |
| PowerFecal | 236.7 ± 35.76 | 50 | 1.95 ± 0.20 | 5.5 ± 3.77c | 1.08 ± 0.629a |
| Zymo | 199.8 ± 30.71 | 50 | 2.04 ± 0.26 | 3.8 ± 1.33c | 0.94 ± 0.277a |