Fig. 1.

C24:0 ceramide accumulates in blood samples of male and female obese and obese-T2DM subjects. A-B) Blood samples were collected from female obese (n = 40) or obese-diabetic (n = 34) and male obese (n = 20) or obese-diabetic subjects (n = 18) as described Materials and Methods. Plasma concentrations were determined by quantitative tandem mass spectrometry. Data are expressed as mean ± SEM. ∗p < 0.05, ∗∗p < 0.001, ∗∗∗p < 0.0001 of obese-T2DM vs. obese following ANOVA and post-hoc Tukey ′s test. Abbreviations, OB: Obese; T2DM: Type 2 diabetes Mellitus.