Table 1. Primers, probes and gene targets for the detection of Bacillus anthracis from bacterial isolates cultured from blood smear samples from wildlife mortalities in Kruger National Park, South Africa by quantitative polymerase chain reaction (qPCR) assays.

Bacillus anthracis protective antigen (BAPA), lethal factor (lef), chromosomal marker (Ba-1 and Genomic island 4: GI4) and the capsule region (capB) were used as molecular markers in this study. FRET stands for fluorescence resonance energy transfer.

Primer/Probe (5′–3′)	Chemistry	Target	Reference
Forward ‐ GTACATCTTCTAGCTGTTGCAA Reverse–ACGTAGGAAGACCGTTGATTA Probe ‐ VIC-CGTTGTTGTGTATTTG-MGB	TaqMan	Ba-1	[41]
Forward–TAAGCCTGCGTTCTTCGTAAATG Reverse–GTTCCCAAATACGTAATGTTGATGAG Probe ‐ NED-TTGCAGCGAATGAT-MGB	TaqMan	capB
Forward–CACTATCAACACTGGAGCGATTCT Reverse–AATTATGTCATCTTTCTTTGGCTCAA Probe ‐ Cy5-AGCTGCAGATTCC-MGB	TaqMan	lef
Forward ‐ GGAGATATTAACAAGAGATGGATTGGA Reverse ‐ CAGTAGGCTTGTCTGCTCTAATAAAATT Probe ‐ FAM-ACATGCCAGCGTTTTTTGCCTCTACACA-BHQ1	Taqman	GI4
Forward–CGGATCAAGTATATGGGAATATAGCAA Reverse ‐ CCGGTTT AGTCGTTT CTAATGGAT BAPA-FL ‐ TGCGGTAACACTT CACTCCAGTTCGA-X BAPA-LCRed 640 - CCTGTATCCACCCTCACTCTT CCATTTT C-P	FRET	pagA with BAPA probe	[3]