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Methods to introduce artificial metalloporphyrins at the level of protein expression. (a) The use of an E. coli strain RP523 with a mutation that renders the membrane heme-permeable. (b) Co-expression of an outer-membrane transporter ChuA with the hemoprotein of interest. (c) This work: in vivo assembly of Ir-CYP119 within E. coli Nissle 1917 that contains a native uptake system for metalloporphyrins.
