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. Author manuscript; available in PMC: 2024 Dec 5.
Published in final edited form as: J Am Chem Soc. 2022 Jan 5;144(2):883–890. doi: 10.1021/jacs.1c10975

Figure 3.

Figure 3.

(a) The turnover numbers for the reaction of phthalan 1 with EDA vs time (h) in whole cells. Reaction conditions: EcN expressing CYP119 mutant, OD600 ~30, phthalan 1 (2 μmol), EDA (16 μmol), DMSO (20 μL), M9-N (300 μL), 30 °C, 1–7 h. The cells were cultivated with 0.1 ppm of Ir(Me)MPIX during protein expression. (b) The C–H activation of 4-chlorophthalan 3b catalyzed by resuspended pellets. The age of cells refers to the length of time for which the cells had been used for catalysis before resuspension. Reaction conditions: resuspended EcN cell pellets, OD600 ~30, 4-chlorophthalan 3b (1 μmol), EDA (8 μmol), DMSO (10 μL), M9-N (300 μL), 30 °C, 2 h. All data are shown as the average from three biological replicates, with error bars indicating 1 standard deviation.