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. Author manuscript; available in PMC: 2024 Dec 5.
Published in final edited form as: J Am Chem Soc. 2022 Jan 5;144(2):883–890. doi: 10.1021/jacs.1c10975

Figure 5.

Figure 5.

C–H bond functionalization of phthalan 1 catalyzed by BL21(DE3) cells co-expressing the potential transporter and CYP119 mutant. The cells were cultivated with 0.1 ppm of Ir(Me)MPIX during protein expression. Reaction conditions: BL21(DE3) expressing CYP119 mutant (C317G, A152L, T213G, P252S, V254A), OD600 ~20, phthalan (2 μmol), EDA (16 μmol), DMSO (20 μL), M9-N (300 μL), 30 °C, 4 h. The relative yield is calculated by normalizing the yield of the reaction in cells co-expressing the E7 transporter and CYP119 as 100%. All data are shown as the average from three biological replicates, with error bars indicating 1 standard deviation.