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. Author manuscript; available in PMC: 2024 Dec 5.
Published in final edited form as: J Am Chem Soc. 2022 Jan 5;144(2):883–890. doi: 10.1021/jacs.1c10975

Scheme 1. Site-Selective C–H Activation Catalyzed by EcN Cells Harboring Ir-CYP119a.

Scheme 1.

aReaction conditions: EcN expressing CYP119 mutant, OD600 ~30, phthalan derivatives (2 μmol), EDA (16 μmol), DMSO (20 μL), M9-N (300 μL), 30 °C, 4 h. The cells were cultivated with 0.1 ppm of Ir(Me)MPIX during protein expression. The heights of bars correspond to the relative amounts of para (blue) and meta (red) products. All data are shown as the average from three biological replicates.