Table 1.

Evaluation of Carbene Insertion into Phthalan 1 Catalyzed by Ir-CYP119 in Nissle 1917 Cells^a

entry	Ir(Me)MPIXb(ppm)	erc	TONd
1	1	70:30	91 ± 11
2	0.5	87:13	273 ± 12
3	0.2	92:8	789 ± 16
4	0.1	92:8	1024 ± 29
5e	0.1	92:8	1314 ± 56
6f	0.1	50:50	56 ± 7

^aReaction conditions: EcN expressing CYP119 mutant (C317G, A152L, T213G, P252S, V254A), OD₆₀₀ ~30, phthalan (2 μmol), EDA (16 μmol), DMSO (20 μl), M9-N (300 μL), 30 °C, 4 h.

^bThe concentrations of Ir(Me)MPIX added to EcN cells during protein expression.

^cThe er (enantiomeric ratio) was determined by chiral HPLC.

^dThe TON (turnover number) was calculated by the number of substrate molecules converted to the product per iridium cofactor added to the cell culture.

^eOD₆₀₀ ~15.

^fThe reaction was performed with EcN cells containing an empty vector pASK-IBA3C under otherwise identical conditions. All data are shown as the average from three biological replicates.