| Other |
Yeast Nitrogen Base with amino acids (YNB) |
BD |
239210 |
Medium used in this study. See section ‘Strains and growth conditions’. |
| Chemical compound, drug |
Lactate |
ChemCruz |
sc-220120B |
|
| Chemical compound, drug |
H2O2
|
Prolabo |
23612.294 |
|
| Chemical compound, drug |
MES |
Sigma-Aldrich |
M3058-100G |
|
| Chemical compound, drug |
MOPS |
Sigma-Aldrich |
M1254-1KG |
|
| Chemical compound, drug |
Acetate |
Sigma-Aldrich |
S2889-1KG |
|
| Chemical compound, drug |
Propionate |
Sigma |
P1880-100G |
|
| Chemical compound, drug |
Butyrate |
Sigma-Aldrich |
B5887-250MG |
|
| Chemical compound, drug |
Calcofluor white (CFW) |
Sigma-Aldrich |
910090-20mL |
|
| Chemical compound, drug |
Congo Red (CR) |
Sigma |
C6277-25G |
|
| Chemical compound, drug |
Tunicamycin |
Sigma |
17765-5MG |
From Streptomyces spp. |
| Chemical compound, drug |
Nikkomycin Z |
Sigma |
N-8028 |
From Streptomyces tendae
|
| Chemical compound, drug |
Caspofungin |
Sigma |
SML0425-5MG |
|
| Other |
Yeast Nitrogen Base without amino acids |
BD |
291940 |
Medium used in this study for biofilm production. See section ‘Strains and growth conditions’. |
| Other |
Breathseal sealing membranes |
Greiner Bio-one |
676051 |
Used in this study for biofilm production. See section ‘Strains and growth conditions’. |
| Commercial assay or kit |
Pierce BCA Protein Assay Kit |
Thermo Scientific |
23225 |
|
| Chemical compound, drug |
Trifluoroacetic acid (TFA) |
Sigma |
8.08260.0101 |
|
| Chemical compound, drug |
Hydrochloric acid (HCl) |
Fisher |
H/1200/PC15 |
|
| Chemical compound, drug |
4-Hydroxybenzhydrazide (PAHBAH) |
Sigma |
H9882-100G |
|
| Chemical compound, drug |
Sodium (meta)periodate (m-IO4Na) |
Sigma-Aldrich |
S1878-25G |
|
| Chemical compound, drug |
Sodium sulfite |
Sigma-Aldrich |
S0505-250G |
|
| Biological sample |
Pustulan |
Elicityl OligoTech |
GLU900-1g |
From Lasallia pustulata
|
| Peptide, recombinant protein |
LamA |
PMID:9168619
|
|
From Thermotoga neapolitana expressed in Escherichia coli
|
| Peptide, recombinant protein |
Endo-β-1,6-glucanase |
PMID:20852022
|
|
From Schizosaccharomyces pombe expressed in Pichia pastoris
|
| Chemical compound, drug |
Sodium azide (NaN3) |
Sigma-Aldrich |
S-8032 |
|
| Chemical compound, drug |
D2O |
Eurisotop |
17247446 |
|
| Other |
CarboPAC-PA1 column |
Thermo Scientific |
035391 |
See sections ‘Cell wall polymers quantification’, ‘Quantification of β-1,6-glucan in AI and AS fractions,’ and ‘Structural characterization of β-1,6-glucan’. |
| Biological sample |
Laminarin |
Sigma |
L9634-5G |
From Laminaria digitata
|
| Other |
Superdex 200 column |
Cytiva |
28990944 |
See sections ‘Structural characterization of β-1,6-glucan’ and ‘PBMC and neutrophil isolation, stimulation by parietal fractions and cytokine quantification’. |
| Biological sample |
Dextran 6 kDa |
Sigma-Aldrich |
31388-25G |
From Leuconostoc spp. |
| Chemical compound, drug |
Dextran 10, 40, 70, and 500 kDa |
Pharmacia Fine Chemicals |
|
|
| Chemical compound, drug |
Glutaraldehyde |
Sigma-Aldrich |
G6257-100ML |
|
| Other |
6 mm type A and B specimen carriers |
Leica |
16770181 and 16770182 |
See section ‘Transmission electron microscopy’. |
| Chemical compound, drug |
Spurr resin |
EMS |
15000 |
|
| Antibody |
Polyclonal anti-β-1,6-glucan (rabbit polyclonal) |
ProteoGenix |
This study |
Pustulan-conjugated BSA used as the antigen IF (1:100) |
| Chemical compound, drug |
Curdlan |
Wako |
034-09901 |
|
| Chemical compound, drug |
Paraformaldehyde |
Electron Microscopy Sciences |
15714 |
|
| Biological sample |
Goat serum |
Sigma |
G9023 |
|
| Other |
Diagnostic Microscope Slides, 8-well 6 mm |
Thermo Scientific |
ER-301B-CE24 |
See section ‘Immunolabeling’. |
| Chemical compound, drug |
Poly-l-lysine |
Sigma-Aldrich |
P4707 |
|
| Antibody |
Monoclonal anti-β-1,3-glucan, named 5 H5 (mouse monoclonal) |
PMID:31022215
|
|
IF (1:250) |
| Antibody |
AlexaFluor 488 goat anti-mouse IgG (goat polyclonal) |
Molecular Probes |
A11029 |
IF (1:200) |
| Antibody |
Fluorescein goat anti-rabbit IgG (goat polyclonal) |
Invitrogen |
F2765 |
IF (1:200) |
| Chemical compound, drug |
Fluoromount-G |
Invitrogen |
00-4958-02 |
|
| Chemical compound, drug |
Ficoll 400 |
Eurobio |
CMSMSL01-0U |
|
| Chemical compound, drug |
RPMI + GlutaMAX medium |
Gibco |
61870044 |
|
| Biological sample |
NHS |
Zenbio |
HSER-ABP100ML |
From human pooled donor |
| Biological sample |
LPS |
Sigma |
L2630 |
From Escherichia coli
|
| Commercial assay or kit |
EasySep Direct Human Neutrophil Isolation Kit |
STEMCELL Technologies |
18001 |
|
| Commercial assay or kit |
Proteome Profiler Human Cytokines Array Kit |
R&D Systems |
ARY005B |
|
| Commercial assay or kit |
DuoSet ELISA kits |
R&D Systems |
DY201, DY278, DY206, DY217B, DY208, DY2037, DY271, DY279, DY210 |
|
| Chemical compound, drug |
BH4Na |
Aldrich |
213462-100G |
|
| Chemical compound, drug |
Glycerol |
Riedel-deHaën |
15523 |
|
| Chemical compound, drug |
AcOH |
Honeywell |
27221-1L |
|
| Other |
Sep-Pak classic C18 cartridge |
Waters |
WAT051910 |
See section ‘PBMC and neutrophil isolation, stimulation by parietal fractions and cytokine quantification’. |
| Peptide, recombinant protein |
BSA |
Sigma-Aldrich |
A3059-100G |
|
| Antibody |
Anti-human C3b (mouse monoclonal) |
Thermo Fisher |
MA1-82814 |
ELISA (1:1000) |
| Antibody |
Peroxidase-conjugated secondary anti-mouse IgG (goat polyclonal) |
Sigma-Aldrich |
A4416 |
ELISA (1:1000) |
| Chemical compound, drug |
TMB |
BioFX |
TMBW-1000-01 |
|
| Recombinant DNA reagent |
pV1093 |
PMID:25977940
|
|
|
| Recombinant DNA reagent |
pSFS2A |
PMID:5474295
|
|
|
| Recombinant DNA reagent |
pV1090 |
PMID:25977940
|
|
|
| Recombinant DNA reagent |
pYM70 |
PMID:20737428
|
|
|
| Peptide, recombinant protein |
Phusion High-Fidelity DNA polymerase |
NEB |
M0530S |
|
| Peptide, recombinant protein |
Q5 polymerase |
NEB |
M0491S |
|
| Commercial assay or kit |
NucleoSpin Gel and PCR Clean-up |
Macherey-Nagel |
740609.250 |
|
| Chemical compound, drug |
Nourseothricin |
Jena Biosciences |
AB-102 |
|
| Commercial assay or kit |
MasterPure Yeast DNA Purification Kit |
Biosearch Technologies |
MPY80200 |
|
| Peptide, recombinant protein |
BP clonase |
Invitrogen |
P/N 56480 |
|
| Peptide, recombinant protein |
LR clonase |
Invitrogen |
P/N 56485 |
|
| Sequence-based reagent |
pDONR207 |
PMID:23049891
|
|
|
| Sequence-based reagent |
Cip10-PACT1-SAT1 |
PMID:29982705
|
|
|
| Strain |
SC5314 |
PMID:6394964
|
|
Clinical blood isolate |
| Strain |
CAF2-1 |
PMID:8349105
|
|
URA3/ura3Δ::imm434
|
| Strain |
CAI4 |
PMID:8349105
|
|
ura3Δ::imm434/ura3Δ::imm434
|
| Strain |
BWP17 |
PMID:10074081
|
|
ura3Δ::imm434/ura3Δ::imm434 his1::hisG/his1::hisG arg4::hisG/arg4::hisG
|
| Strain |
SN152 |
PMID:15701792
|
|
arg4Δ/arg4Δ leu2Δ/leu2Δ his1Δ/his1Δ URA3/ura3Δ::imm434 IRO1/iro1Δ::imm434
|
| Strain |
cwh41Δ/Δ
|
PMID:17933909
|
|
Same as CAI4 but cwh41Δ::dp1200/cwh41Δ::dp1200 RPS1/rps1Δ::CIp10
|
| Strain |
rot2Δ/Δ
|
PMID:17933909
|
|
Same as CAI4 but rot2Δ::dp1200/rot2Δ::dp1200 RPS1/rps1Δ::CIp10
|
|
Strain
|
kre5Δ/Δ
|
PMID:20543849
|
|
Same as SN152 but kre5Δ::leu2/kre5Δ::his1
|
|
Strain
|
kre6Δ/Δ
|
This study |
|
kre6Δ::HygB/kre6Δ::HygB
|
| Strain |
kre62Δ/Δ
|
This study |
|
kre62Δ::FRT/kre62Δ::FRT
|
| Strain |
skn2Δ/Δ
|
This study |
|
skn2Δ::FRT/skn2Δ::FRT |
| Strain |
skn1Δ/Δ
|
This study |
|
skn1Δ::FRT/skn1Δ::FRT
|
| Strain |
kre6/skn1Δ/Δ
|
This study |
|
kre6Δ::HygB/kre6Δ::HygB skn1Δ::FRT/skn1Δ::FRT
|
| Strain |
kre6/kre62/skn2/skn1Δ/Δ
|
This study |
|
kre6Δ::HygB/kre6Δ::HygB kre62Δ::FRT/kre62Δ::FRT skn2Δ::FRT/skn2Δ::FRT skn1Δ::FRT/skn1Δ::FRT
|
| Strain |
kre6/kre62/skn2/skn1Δ/Δ+P
ACT1
-KRE6
|
This study |
|
kre6Δ::HygB/kre6Δ::HygB kre62Δ::FRT/kre62Δ::FRT skn2Δ::FRT/skn2Δ::FRT skn1Δ::FRT/skn1Δ::FRT RPS1/RPS1::CIpSAT1-P
ACT1
-KRE6
|
| Strain |
kre1Δ/Δ
|
Provided by Mathias Richard |
|
Same as BWP17 but kre1Δ::arg4/kre1Δ::his1
|
|
Strain
|
P
MRP1
-CHS1/chs1Δ
|
PMID:11251855
|
|
Same as CAI4 but chs1Δ::hisG/chs1Δ:pSK-URA3-PMRP1-CHS1
|
|
Strain
|
chs2Δ/Δ
|
PMID:8636047
|
|
Same as CAF2-1 but chs2Δ::hisG/chs2Δ::hisG-URA3-hisG
|
| Strain |
chs3Δ/Δ
|
PMID:7479842
|
|
Same as CAF2-1 but chs3-2::hisG/chs3-3::hisG-URA3-hisG
|
| Strain |
mnt1/mnt2Δ/Δ
|
PMID:15519997
|
|
Same as CAF2-1 but mnt1-mnt2Δ::hisG/mnt1-mnt2Δ::hisG-URA3-hisG
|
| Strain |
mnn2/22/21/23/24/26Δ/Δ
|
PMID:23633946
|
|
Same as CAF2-1 but mnn2Δ::dpl200/mnn2Δ::dpl200 mnn22Δ::dpl200/mnn2Δ::dpl200 mnn23Δ::dpl200/mnn23Δ::dpl200 mnn24Δ::dpl200/mnn24Δ::dpl200 mnn26Δ::dpl200/mnn26Δ::dpl200 mnn21Δ::dpl200/mnn21Δ::dpl200
|
| Strain |
mnn9Δ/Δ
|
PMID:10601199
|
|
Same as CAF2-1 mnn9Δ::hisG/mnn9Δ::hisG Δura3Δ::imm434/ura3Δ::imm434
|
| Strain |
och1Δ/Δ
|
PMID:20543849
|
|
Same as SN152 but och1Δ::leu2/och1Δ::his1
|
| Strain |
fks1Δ
|
PMID:30370375
|
|
SC5314, but fks1/fks1Δ
|
| Strain |
phr1Δ/Δ
|
PMID:7823929
|
|
Same as BWP17 but phr1Δ::hisG/phr1Δ
|
| Strain |
phr2Δ/Δ
|
PMID:9315654
|
|
Same as BWP17 but phr2Δ::hisG/phr2Δ::hisG-URA3-hisG
|
| Sequence-based reagent |
SNR52/F |
PMID:27340698
|
PCR primers |
See sequence in Supplementary file 2
|
| Sequence-based reagent |
sgRNA/R |
PMID:27340698
|
PCR primers |
See sequence in Supplementary file 2
|
| Sequence-based reagent |
SNR52/N |
PMID:27340698
|
PCR primers |
See sequence in Supplementary file 2
|
| Sequence-based reagent |
sgRNA/N |
PMID:27340698
|
PCR primers |
See sequence in Supplementary file 2
|
| Sequence-based reagent |
CaCas9/F |
PMID:27340698
|
PCR primers |
See sequence in Supplementary file 2
|
| Sequence-based reagent |
CaCas9/R |
PMID:27340698
|
PCR primers |
See sequence in Supplementary file 2
|
| Sequence-based reagent |
SNR52/R/SKN1 |
This study |
PCR primers |
See sequence in Supplementary file 2
|
| Sequence-based reagent |
SNR52/R/SKN2 |
This study |
PCR primers |
See sequence in Supplementary file 2
|
| Sequence-based reagent |
SNR52/R/KRE62 |
This study |
PCR primers |
See sequence in Supplementary file 2
|
| Sequence-based reagent |
SNR52/R/KRE6 |
This study |
PCR primers |
See sequence in Supplementary file 2
|
| Sequence-based reagent |
sgRNA/F/SKN1 |
This study |
PCR primers |
See sequence in Supplementary file 2
|
| Sequence-based reagent |
sgRNA/F/SKN2 |
This study |
PCR primers |
See sequence in Supplementary file 2
|
| Sequence-based reagent |
sgRNA/F/KRE62 |
This study |
PCR primers |
See sequence in Supplementary file 2
|
| Sequence-based reagent |
sgRNA/F/KRE6 |
This study |
PCR primers |
See sequence in Supplementary file 2
|
| Sequence-based reagent |
SAT1FLP/F/SKN1 |
This study |
PCR primers |
See sequence in Supplementary file 2
|
| Sequence-based reagent |
SAT1FLP/F/SKN2 |
This study |
PCR primers |
See sequence in Supplementary file 2
|
| Sequence-based reagent |
SAT1FLP/F/KRE62 |
This study |
PCR primers |
See sequence in Supplementary file 2
|
| Sequence-based reagent |
HygR/F/KRE6 |
This study |
PCR primers |
See sequence in Supplementary file 2
|
| Sequence-based reagent |
SAT1FLP/R/SKN1 |
This study |
PCR primers |
See sequence in Supplementary file 2
|
| Sequence-based reagent |
SAT1FLP/R/SKN2 |
This study |
PCR primers |
See sequence in Supplementary file 2
|
| Sequence-based reagent |
SAT1FLP/R/KRE62 |
This study |
PCR primers |
See sequence in Supplementary file 2
|
| Sequence-based reagent |
hygR/R/KRE6 |
This study |
PCR primers |
See sequence in Supplementary file 2
|
| Sequence-based reagent |
Flanking_F/SKN1 |
This study |
PCR primers |
See sequence in Supplementary file 2
|
| Sequence-based reagent |
Flanking_F/SKN2 |
This study |
PCR primers |
See sequence in Supplementary file 2
|
| Sequence-based reagent |
Flanking_F/KRE62 |
This study |
PCR primers |
See sequence in Supplementary file 2
|
| Sequence-based reagent |
Flanking_R/SKN1 |
This study |
PCR primers |
See sequence in Supplementary file 2
|
| Sequence-based reagent |
Flanking_R/SKN2 |
This study |
PCR primers |
See sequence in Supplementary file 2
|
| Sequence-based reagent |
Flanking_R/KRE62 |
This study |
PCR primers |
See sequence in Supplementary file 2
|
| Sequence-based reagent |
screenKre6/F |
This study |
PCR primers |
See sequence in Supplementary file 2
|
| Sequence-based reagent |
screenKre6/R |
This study |
PCR primers |
See sequence in Supplementary file 2
|
| Sequence-based reagent |
CIpUL |
This study |
PCR primers |
See sequence in Supplementary file 2
|
| Sequence-based reagent |
CIpSAT |
This study |
PCR primers |
See sequence in Supplementary file 2
|
| Sequence-based reagent |
KRE6-FWD |
This study |
PCR primers |
See sequence in Supplementary file 2
|
| Sequence-based reagent |
KRE6-REV |
This study |
PCR primers |
See sequence in Supplementary file 2
|
