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. 2024 Nov 22;15:1450124. doi: 10.3389/fimmu.2024.1450124

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Copyright © 2024 He, Wang, Tan, Hu, Ma, Mi, Li, Zhang, Du, Zhang, Li, Jiao and Zhu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Construction of fusion protein LT33 and LT28. Expression, purification, and analysis of fusion protein LT33 and LT28. (A) The 3D structure of ESAT6 (red), CFP10 (blue), Rv1738 (green), and the fusion protein LT33. (B) Purification of LT33 verified with polyacrylamide gel electrophoresis. E. coli BL21 expressing LT33 lysate (lane 1), purification of LT33 (lane 2) (C) Purified LT33 was verified by immunoblot using anti-ESAT6 serum. Negative control (lane 1), mouse polyclonal anti-ESAT6 (lane 2), M, molecular weight. (D) The 3D structure of MPT64 (red), Rv1978 (blue), Rv2645 (green), and the fusion protein LT28. (E) Purification of LT28 verified with polyacrylamide gel electrophoresis. Purification of LT28 (lane 1). (F) Purified LT28 was verified by immunoblot with anti-MPT64 serum. Mouse polyclonal anti-MPT64 (lane 1), negative control (lane 2). M, molecular weight.