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. 2024 Nov 22;15:1450124. doi: 10.3389/fimmu.2024.1450124

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Copyright © 2024 He, Wang, Tan, Hu, Ma, Mi, Li, Zhang, Du, Zhang, Li, Jiao and Zhu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The immunogenicity of LT28 in mice. At 12 weeks after the last immunization, the mice were stimulated with a single antigen (MPT64, Rv1978, and Rv2645 peptide) in vivo at 3 days before the mice were euthanized. Then, the splenic lymphocytes were separated and stimulated with a single antigen (MPT64, Rv1978, and Rv2645) in vitro for 12h. Intracellular cytokine staining was analyzed using flow cytometry. (A) Flow cytometric analysis of IFN-γ-producing CD4⁺ T cells by re-stimulation. (B) Statistical analysis of the proportion of IFN-γ-producing CD4⁺ T cells. Results are presented as means ± SD, n = 4. ***p < 0.005.