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The protective efficacy of LT33 and LT28 challenged with the H37Ra strain. At 12 weeks after the last immunization, the C57BL/6 mice were intravenously challenged with the M. tuberculosis H37Ra strain. The protective efficacy was measured by detecting the bacteria load in lung tissues and spleen tissues. Mice were euthanized and the bacterial burden (H37Ra) was measured in the spleens (A) and lungs (B). All data were shown as means ± SD, n = 3–6. *p < 0.05; ***p <0.001.
